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ferritin heavy chain fhc antibody  (ZenBio)

 
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    ZenBio ferritin heavy chain fhc antibody
    Ferritin Heavy Chain Fhc Antibody, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ferritin+heavy+chain+fhc+antibody/ferritin+heavy+chain+fhc+antibody/pm38035662-127-45-53
    Average 90 stars, based on 1 article reviews
    ferritin heavy chain fhc antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Molecular mechanism of interleukin-17A regulating airway epithelial cell ferroptosis based on allergic asthma airway inflammation.
    Article Snippet: A 12 % sodium dodecyl sulfate-polyacrylamide gel (Yamei, China) was applied for protein separation; then proteins were transferred on the polyvinylidene difluoride (PVDF) membranes (Millipore, Germany). .. Next, membranes were subjected to blocking and overnight incubation using primary antibodies of prostaglandin-endoperoxide synthase 2 (PTGS2, Cat #: ab179800, 1:20000, Abcam), solute carrier family 7 member 11 (SLC7A11 (xCT), Cat #: 382036, 1:1000, ZENBIO), fatty acid-CoA ligase 4 (FACL4, Cat #: ab155282, 1:20000, Abcam), ferritin heavy chain (FHC, Cat #: R23306, 1:1000, ZENBIO), glutathione peroxidase 4 (GPX4, Cat #: 381958, 1:1000, ZENBIO), tumor necrosis factor-α (TNF-α, Cat #: AF7014, 1:1000, Affinity Biosciences, China), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB p65, Cat #: AF7014, 1:1000, Affinity Biosciences, China), phospho–NF–kB p65 (Ser536) (Cat #: AF2006, 1:1000, Affinity Biosciences, China), and β-actin (Cat #:4970, 1:1000, Cell Signaling Technology) at 4 ◦C. .. Membranes were later rinsed three times using TBST, followed by incubation using goat anti-rabbit IgG (H + L) (Cat #: S0001, 1:5000, Affinity Biosciences, China) or goat anti-mouse IgG (H + L) HRP (Cat #: S0002, 1:5000, Affinity Biosciences, China) for 2 h at RT.

    Incubation:

    Article Title: Molecular mechanism of interleukin-17A regulating airway epithelial cell ferroptosis based on allergic asthma airway inflammation.
    Article Snippet: A 12 % sodium dodecyl sulfate-polyacrylamide gel (Yamei, China) was applied for protein separation; then proteins were transferred on the polyvinylidene difluoride (PVDF) membranes (Millipore, Germany). .. Next, membranes were subjected to blocking and overnight incubation using primary antibodies of prostaglandin-endoperoxide synthase 2 (PTGS2, Cat #: ab179800, 1:20000, Abcam), solute carrier family 7 member 11 (SLC7A11 (xCT), Cat #: 382036, 1:1000, ZENBIO), fatty acid-CoA ligase 4 (FACL4, Cat #: ab155282, 1:20000, Abcam), ferritin heavy chain (FHC, Cat #: R23306, 1:1000, ZENBIO), glutathione peroxidase 4 (GPX4, Cat #: 381958, 1:1000, ZENBIO), tumor necrosis factor-α (TNF-α, Cat #: AF7014, 1:1000, Affinity Biosciences, China), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB p65, Cat #: AF7014, 1:1000, Affinity Biosciences, China), phospho–NF–kB p65 (Ser536) (Cat #: AF2006, 1:1000, Affinity Biosciences, China), and β-actin (Cat #:4970, 1:1000, Cell Signaling Technology) at 4 ◦C. .. Membranes were later rinsed three times using TBST, followed by incubation using goat anti-rabbit IgG (H + L) (Cat #: S0001, 1:5000, Affinity Biosciences, China) or goat anti-mouse IgG (H + L) HRP (Cat #: S0002, 1:5000, Affinity Biosciences, China) for 2 h at RT.



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    Image Search Results


    Antibodies used for Western blots

    Journal: Journal of Neuroimmune Pharmacology

    Article Title: The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain

    doi: 10.1007/s11481-023-10082-x

    Figure Lengend Snippet: Antibodies used for Western blots

    Article Snippet: Ferritin heavy chain (FHC) , Cell Signaling Technology 3998S , RRID:AB_1903974 , 1:1000.

    Techniques: Western Blot, Diagnostic Assay

    Pharmacological inhibition of DMT1. Western blot showing ferritin heavy chain (FHC) protein levels in primary neurons treated with either morphine (1 µM, 24 h), ebselen (10 µM, 24.5 h), or pre-treated with ebselen for 0.5 h followed by morphine treatment as above. Ferric ammonium citrate (FAC) (25 µM, 24 h) used as a positive control to increase FHC protein level. Vehicle controls include ddH2O for morphine and 0.2% DMSO for ebselen. FHC expression quantified in adjacent graph. GAPDH antibody shows protein loading. N = 5, one-way ANOVA with Dunnett’s post hoc, *p = 0.0315, **** p < 0.001

    Journal: Journal of Neuroimmune Pharmacology

    Article Title: The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain

    doi: 10.1007/s11481-023-10082-x

    Figure Lengend Snippet: Pharmacological inhibition of DMT1. Western blot showing ferritin heavy chain (FHC) protein levels in primary neurons treated with either morphine (1 µM, 24 h), ebselen (10 µM, 24.5 h), or pre-treated with ebselen for 0.5 h followed by morphine treatment as above. Ferric ammonium citrate (FAC) (25 µM, 24 h) used as a positive control to increase FHC protein level. Vehicle controls include ddH2O for morphine and 0.2% DMSO for ebselen. FHC expression quantified in adjacent graph. GAPDH antibody shows protein loading. N = 5, one-way ANOVA with Dunnett’s post hoc, *p = 0.0315, **** p < 0.001

    Article Snippet: Ferritin heavy chain (FHC) , Cell Signaling Technology 3998S , RRID:AB_1903974 , 1:1000.

    Techniques: Inhibition, Western Blot, Positive Control, Expressing